Profiling protease activities by dynamic proteomics workflows
نویسندگان
چکیده
منابع مشابه
In-cell selectivity profiling of serine protease inhibitors by activity-based proteomics.
Activity-based proteomics is a methodology that is used to quantify the catalytically active subfraction of enzymes present in complex mixtures such as lysates or living cells. To apply this approach for in-cell selectivity profiling of inhibitors of serine proteases, we designed a novel activity-based probe (ABP). This ABP consists of (i) a fluorophosphonate-reactive group, directing the probe...
متن کاملIn-cell Selectivity Profiling of Serine Protease Inhibitors by Activity-based Proteomics*□S
Activity-based proteomics is a methodology that is used to quantify the catalytically active subfraction of enzymes present in complex mixtures such as lysates or living cells. To apply this approach for in-cell selectivity profiling of inhibitors of serine proteases, we designed a novel activity-based probe (ABP). This ABP consists of (i) a fluorophosphonate-reactive group, directing the probe...
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ژورنال
عنوان ژورنال: PROTEOMICS
سال: 2012
ISSN: 1615-9853
DOI: 10.1002/pmic.201100399